Evaluation of Antioxidant Activities of Fractions of Zea Mays Husk Extract Against CCL4-Induced Oxidative Stress in Wistar Rats

Author

Abiodun Abdulahi Jimoh, Usman Wali, Mohammed Haruna Yeldu, Abdullahi Yahya Abbas, Ibrahim Zubairu Wasagu, Danjuma Mohammed, Aliu Oriyomi Jimoh

Abstract

 We investigated the effects of fractions of maize husk extract on CCL4 induced oxidative stress. Forty (40) Wistar rats of eight (8) groups with five (5) rats each and CCL4 induced oxidative stress was established in group 2 to 8 followed by treatment with the fractions of maize husk extract. The group 1, 2, and 3 serves as normal, oxidative stress control, silymarin control while group 4, 5, 6, 7 and 8 represents 200mg/kg of aqueous, hexane, ethylaceteta, butanol and methanol fractions of maize husk followed by the measurements of MDA, CAT and SOD, renal function test, glucose level and phytochemical screening. The fractions significantly decreased MDA and increased CAT, SOD and glucose level (p< 0.05) compared with CCL4-induce. The extract significantly (p < 0.05) reduce serum levels of creatinine, urea and chloride compared with CCL4 induced group. Histology of kidney revealed significant reductions in degenerative changes observed in the treated rats compared to untreated rats. The phytochemical screening of hexane fraction reveal presence of steroids, phenols, tetracontain and 5-Octadecene. The results showed that the fractions of Zea mays has antioxidative capacities which may be due to the antioxidant activities of their phytochemical constituents.

Keywords

Histology, Silymarin, Phytochemical, phenols

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References

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